Histone 3 anticorps (pThr45)

N° du produit ABIN6972078

L’anticorps Lapin Polyclonal anti-Histone 3 a été validé pour WB et DB. Il convient pour détecter Histone 3 dans des échantillons de Humain et Saccharomyces cerevisiae.

Aperçu rapide pour Histone 3 anticorps (pThr45) (ABIN6972078)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain, Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Dot Blot (DB)

Détail du produit anti-Histone 3 anticorps

Épitope: pThr45

Attributs du produit: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of histone H2A, histone H2B, histone H3 and histone H4). Histones are subject to a number of post-translational modifications, including acetylation, phosphorylation and methylation. In budding yeast, H3 Thr45 phosphorylation occurs specifically during DNA replication, and is catalyzed by the Cdc7-Dbf4 kinase. Yeast that lack threonine 45 exhibit defects in replication, suggesting that phosphorylation of Thr45 is associated with DNA replication. Threonine 45 lies within a conserved region of histone H3 that makes critical contacts with DNA when assembled into chromatin. It is located at the points of entry and exit of DNA on the nucleosome, and phosphorylation may influence histone-DNA interactions within the nucleosome. Histone H3T45ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot, it has been shown to react with Budding Yeast and Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Affinity Purified

Immunogène: This Histone H3 phospho Thr45 antibody was raised against a peptide containing phospho Thr45 of human histone H3.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone H3

Poids moléculaire: 17 kDa

NCBI Accession: NP_003522